Mode of Action
The cytochrome oxidase is an enzyme of the iron porphyrine group which is very widely distributed in nature. It oxidizes the reduced cytochrome c and is thus transformed itself into the reduced and inactive form. Through transfer of the electrons to molecular oxygen the reduced cytochrome oxidase is transformed again into the active form. In the presence of molecular oxygen the cytochrome oxidase/ cytochrome c-system can reduce a whole series of organic substances, among them the socalled NaDi reagent (1-naphthol +dimethylparaphenylene diamine) with formation of the condensation molecule indophenol blue.
This reaction is used for the classification and identification of bacteria.
The reaction zone of a teststrip contains:
N,N-dimethyl-1,4-phenylene diammonium chloride 0.1 µmol;
1-naphthol 1.0 µmol.
The separate colonies grown on a culture medium or, in the case of pure cultures, an inoculation loop full are being tested. Instead with bacterial mass the reaction may also be performed with a dense bacterial suspension.
See expiry date.
Only remove the amount of strips needed at the time!
Do not touch the reaction zones of the test strips.
Close receptacle firmly immediately after use. The strips with deep brown coloured reaction zone are unusable. Please store at the specified temperature.
Store tightly closed in a cool dry place at +2 °C to +8 °C.
The test strip is to be removed safety after use like bacteria containing material. This may be done by burning, autoclaving or by placing into a 5 to 6% desinfectant solution – for at least 6 hours.
With an inoculating loop take a separate, wellgrown colony from the culture medium.
Apply the colony to the reaction zone and spread with the inoculating loop.
After approx. 20 to 60 seconds compare with the colour scale