This medium complies with ISO 21528-1 the specifications of the Eiprodukte-Verordnung (German Egg Product Regulations) (1975) and the European Pharmacopeia II.
Mode of Action
The undesired, accompanying bacterial flora is almost completely inhibited by brilliant green and ox bile. Dextrose
favours the growth of all Enterobacteriaceae. The strong buffering capacity of the culture medium prevents the formed acid from killing the culture.
Typical Composition (g/litre)
Peptones 10.0; D(+)glucose 5.0; ox bile, dried 20.0; brilliant green 0.0135; di-sodium hydrogen phosphate-dihydrate 8.0; potassium dihydrogen phosphate 2.0.
Suspend 45 g/litre, dispense into test tubes and autoclave under mild conditions (5 min at 121 °C), or heat at 100 °C for 30minutes in a waterbath or flowing steam.
pH: 7.2 ± 0.2 at 25 °C.
The prepared broth is clear and green.
Experimental Procedure and Evaluation
Inoculate the broth with the sample material.
Incubation: 24-48 hours at 35 °C aerobically.
If the medium shows bacterial growth, transfer some of the resulting material to selective culture media