FRASER Broth (base)

R3,882.25

8 in stock (can be backordered)

For the selective enrichment of Listeria in the 2-step method acc. to D.G.AL. and ISO 11290-1 (1996)

For items in stock, allow 5-7 working days delivery period within South Africa

and 7-10 working days for orders within Southern Africa

Description

sterility: non-sterile

form: powder

shelf life: limited shelf life, expiry date on the label

composition
casein enzymic hydrolysate, 5.0 g/L
disodium phosphate dihydrate, 12.0 g/L
esculin, 1.0 g/L
lithium chloride, 3.0 g/L
meat extract, 5.0 g/L
peptic digest of animal tissue, 5.0 g/L
potassium dihydrogen phosphate, 1.35 g/L
sodium chloride, 20.0 g/L
yeast extract, 5.0 g/L

technique(s)
microbiological culture: suitable

final pH
7.2±0.2 (25 °C)

application(s)
agriculture
environmental
food and beverages
pathogen testing
sample preparation
microbiology

suitability
selective and differential for Listeria spp.

DESCRIPTION
Application
Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds.

Preparation Note
Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Additional information

Weight 0.5 kg

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