Mode of Action
The broth is rich in nutrients and produces high resuscitation rates for sublethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.
Dissolve 25.5 g in 1 l of purified water. If desired dispense into smaller vessels and autoclave 15 min at 121 °C.
The prepared medium is clear and yellowish. The pH value at 25 °C is in the range of 6.8-7.2.
Experimental Procedure and Evaluation
Depend on the purpose for which the medium is used. Incubate the inoculated broth under aerobic conditions, e.g. acc. to EN ISO 6579 36-38 °C for 16-20 h, acc. to EN ISO/FDIS 6579-1 at 34-38 °C for 16-20 h.
Transfer material from the resulting culture to a selective enrichment culture medium following the method given by the appropriate standard.
According to EN ISO/FDIS 6579-1, it is permissible to store the pre-enriched sample after incubation at +2 to +8 °C for a maximum of 72 h.