This broth conforms with the German Pharmacopeia DAB10 (1991) guidelines.
Mode of Action
The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive microorganisms in particular.
Typical Composition (g/litre)
Potassium dihydrogen phosphate 3.56; di-sodium hydrogen phosphate dihydrate 7.23; sodium chloride 4.3; peptone from pepsin-digested meat 1.0.
pH: 7.0 ± 0.2 at 25 °C.
Suspend in demineralized water to give a concentration of 16.1 g/litre, aliquot into smaller vessels if required and, if necessary, add 1-10 ml Tween® 20 or 80 per litre of culture medium.
Autoclave for 15 minutes at 121 °C.
The medium is clear and yellowish.
Application and Evaluation
Samples for colony count and for the detection of Pseudomonas aeruginosa and Staphylococcus aureus should be diluted according to DAB 10.
Incubation: 24 h at 35 °C aerobically