This culture medium can be used without blood e.g. for setting up blood cultures (UPDYKE 1970) and as a base for preparing special culture media
\nThe medium complies with the recommendations of APHA (1992) for the examination of foodstuffs.<\/p>\n
Principle<\/strong> Mode of Action<\/strong> TARSHIS and FRISH (1951) recommended addition of 1% glycerol and 25 % human blood when isolating tubercle bacilli from sputum, since recognizable mycobacteria colonies grow from even minimal amounts of sample material. Typical Composition (g\/litre)<\/strong> Also to be added: <\/p>\n non-sterile<\/span><\/p>\n<\/div>\n<\/div>\n form<\/span><\/p>\n<\/div>\n<\/div>\n powder<\/span><\/p>\n<\/div>\n<\/div>\n limited shelf life, expiry date on the label<\/span><\/p>\n<\/div>\n<\/div>\n agar, 15\u00a0g\/L<\/span> NutriSelect\u00ae<\/sup>\u00a0Plus<\/span><\/p>\n<\/div>\n<\/div>\n microbe id | specific enzyme detection: suitable<\/span> 7.3\u00b10.2 (25\u00a0\u00b0C)<\/span><\/p>\n<\/div>\n<\/div>\n agriculture Aeromonas<\/i>\u00a0spp.<\/span> Not In Stock (Back Order Delivery: 4-6 Weeks)<\/b><\/span><\/p>\n","protected":false},"featured_media":2538,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[125,116,117,17,98],"product_tag":[],"class_list":{"0":"post-2537","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-food-and-beverage-qa-lab","7":"product_cat-medical-lab","8":"product_cat-agriculture","9":"product_cat-culture-media","10":"product_cat-microbiology-reagent","12":"first","13":"onbackorder","14":"shipping-taxable","15":"purchasable","16":"product-type-simple"},"yoast_head":"\n
\nMicrobiological method<\/p>\n
\nThis culture medium represents a rich nutrient base, which provides optimal growth conditions for all relevant microorganisms. The pH value of 6.8 stabilizes the red blood corpuscles and favours the formation of clear haemolysis zones (NORTON 1932). Fresh, defibrinated sheep blood is most suitable for determining haemolysis forms.
\nBoiled blood agar (“chocolate agar”) is an extremely rich culture medium and can be prepared by heating after the blood has been added.
\nIf the culture medium base is to be used without blood, the pH should, however, be adjusted to 7.2 to 7.4 since most bacterial colonies appear somewhat earlier and grow better in a slightly alkaline medium.<\/p>\n
\nHOSTY et al. (1953) reported, however, that 0.1 % glycerol and 2.5 % human blood together with 100 IU\/mol of penicillin as a selective agent are sufficient. According to SONDAG et al. (1977) and BLACK a. VAN BUSKIRK (1973), addition of 5 mg\/l gentamicin (e.g. 0.1 ml gentamicin solution) to blood agar permits selective cultivation of Streptococcus pneumoniae and other Streptococci as well as bacterioides, Clostridium and
\nyeasts.
\nFor the selective cultivation of Aeromonas MISHRA et al. (1987) recommend an ampicillin sheep blood agar (ASBA 30).<\/p>\n
\nNutrient substrate (heart extract and peptones) 20.0;
\nsodium chloride 5.0:
\nagar-agar 15.0.<\/p>\n
\nBlood 50-80 ml.<\/p>\nPROPERTIES<\/h2>\n<\/div>\n
sterility<\/h3>\n<\/div>\n
shelf life<\/h3>\n<\/div>\n
composition<\/h3>\n<\/div>\n
\nmeat extract, 10\u00a0g\/L<\/span>
\npeptone, 10\u00a0g\/L<\/span>
\nsodium chloride, 5\u00a0g\/L<\/span><\/p>\n<\/div>\n<\/div>\nmanufacturer\/tradename<\/h3>\n<\/div>\n
technique(s)<\/h3>\n<\/div>\n
\nmicrobiological culture: suitable<\/span><\/p>\n<\/div>\n<\/div>\nfinal pH<\/h3>\n<\/div>\n
application(s)<\/h3>\n<\/div>\n
\nclinical testing
\nenvironmental
\nfood and beverages<\/span>
\nmicrobiology<\/span><\/p>\n<\/div>\n<\/div>\nsuitability<\/h3>\n<\/div>\n
\nHaemophilus<\/i>\u00a0spp.<\/span>
\nPneumococcus<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Bacillus cereus<\/i><\/span>
\nnonselective and differential for\u00a0Bacillus<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Bordetella<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Candida<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Clostridium<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Corynebacterium<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Enterococcus<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Escherichia coli<\/i><\/span>
\nnonselective and differential for\u00a0Listeria<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Neisseria<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Pseudomonas<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Salmonella<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Shigella<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Staphylococcus<\/i>\u00a0spp.<\/span>
\nnonselective and differential for\u00a0Streptococcus<\/i>\u00a0spp.<\/span>
\nnonselective and differential for bacteria (General Media)<\/span>
\nnonselective and differential for coliforms<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"