{"id":3796,"date":"2023-01-19T04:27:01","date_gmt":"2023-01-19T04:27:01","guid":{"rendered":"https:\/\/idealmedical.co.za\/wp\/?post_type=product&p=3796"},"modified":"2025-06-16T02:28:16","modified_gmt":"2025-06-16T02:28:16","slug":"folin-ciocalteu%e2%80%b2s-phenol-reagent-2n","status":"publish","type":"product","link":"https:\/\/idealmedical.co.za\/wp\/product\/folin-ciocalteu%e2%80%b2s-phenol-reagent-2n\/","title":{"rendered":"Folin & Ciocalteu\u2032s phenol reagent 2N"},"content":{"rendered":"
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General description<\/h3>\n
Folin & Ciocalteu\u2032s phenol reagent is most commonly used in the Lowry method for determining protein concentration.It has also been used for the quantification of total phenolics.<\/div>\n
In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
\nstabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg\/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
\nIn the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine..
\nMany modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
\nFolin & Ciocalteu\u2032s phenol reagent can also be used as a spray reagent in chromatographic procedures.<\/div>\n
<\/div>\n<\/div>\n
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Application<\/h3>\n
Folin & Ciocalteu\u2032s phenol reagent is used for the estimation of total phenolic content in samples.<\/div>\n
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Packaging<\/h3>\n
100, 500 mL in glass bottle<\/div>\n
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PROPERTIES<\/h2>\n<\/div>\n
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form<\/h3>\n<\/div>\n
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liquid<\/span><\/p>\n<\/div>\n<\/div>\n

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<\/div>\n
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concentration<\/span><\/p>\n<\/div>\n<\/div>\n

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2\u00a0N<\/span><\/p>\n<\/div>\n<\/div>\n

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color<\/h3>\n<\/div>\n
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clear yellow<\/span><\/p>\n<\/div>\n<\/div>\n

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pH<\/h3>\n<\/div>\n
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<0.5 (20\u00a0\u00b0C)<\/span><\/p>\n<\/div>\n<\/div>\n

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density<\/h3>\n<\/div>\n
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1.240\u00a0g\/cm3<\/sup>\u00a0at 20\u00a0\u00b0C<\/span><\/p>\n<\/div>\n<\/div>\n

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suitability<\/h3>\n<\/div>\n
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suitable for determination of total protein by Lowry method<\/span><\/p>\n<\/div>\n<\/div>\n

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application(s)<\/h3>\n<\/div>\n
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diagnostic assay manufacturing
\nhematology
\nhistology<\/span><\/p>\n<\/div>\n<\/div>\n

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storage temp.<\/h3>\n<\/div>\n
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room temp<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

General description Folin & Ciocalteu\u2032s phenol reagent is most commonly used in the Lowry method for determining protein concentration.It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution stabilized with sodium potassium tartrate). Addition of the phenol reagent […]<\/p>\n","protected":false},"featured_media":3794,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[121,122,20],"product_tag":[],"class_list":{"0":"post-3796","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-hematology","7":"product_cat-histology","8":"product_cat-lab-reagent","10":"first","11":"instock","12":"shipping-taxable","13":"purchasable","14":"product-type-variable"},"yoast_head":"\nFolin & Ciocalteu\u2032s phenol reagent 2N - ideal solutions<\/title>\n<meta name=\"description\" content=\"Folin & Ciocalteu\u2032s phenol reagent, used for the estimation of total phenolic content in samples. 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