{"id":481,"date":"2020-06-28T14:42:38","date_gmt":"2020-06-28T14:42:38","guid":{"rendered":"https:\/\/idealmedical.co.za\/wp\/?post_type=product&p=481"},"modified":"2025-05-30T02:32:18","modified_gmt":"2025-05-30T02:32:18","slug":"xld-agar-90mm-li-usp-contact-plates","status":"publish","type":"product","link":"https:\/\/idealmedical.co.za\/wp\/product\/xld-agar-90mm-li-usp-contact-plates\/","title":{"rendered":"XLD Agar 90 mm settle plates – LI EP+USP 30ml"},"content":{"rendered":"

Ten settle plates each with a diameter of 90 mm are single-bagged in transparent, hydrogen peroxide impermeable sleeves (non-irradiated). The sleeves consist of polypropylene with a barrier of PE-EVOHPE.
\nThe formulation of the basic medium is prepared according to the recommendations of the current European and United States Pharmacopoeia (EP, 2.6.12. and USP, 61).<\/p>\n

Mode of Action<\/strong>
\nDegradation of xylose, lactose and sucrose to acid causes phenol red to change its color to yellow.
\nProduction of hydrogen sulfide is indicated by thiosulfate and iron(III) salt, which react to form a precipitate of black iron sulfide in the colonies.
\nBacteria which decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH.
\nThese reactions can proceed simultaneously or successively, this may cause the pH indicator to exhibit various shades of color or it may change its color from yellow to red on prolonged incubation. The culture medium is weakly inhibitory.<\/p>\n

Typical Composition<\/strong>
\nYeast Extract\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a03 g\/l
\nL-Lysine\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a05 g\/l
\nSucrose (Saccharose)\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 7.5 g\/l
\nXylose\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a03.5 g\/l
\nLactose Monohydrate\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a07.5 g\/l
\nSodium Deoxycholate\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 2.5 g\/l
\nAmmonium Iron(III) Citrate\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 0.8 g\/l
\nSodium Thiosulfate\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 6.8 g\/l
\nNaCl\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a05 g\/l
\nPhenol Red\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 80 mg\/l
\nAgar\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a013.5 g\/l<\/p>\n

The appearance of the medium is clear and red, possibly with white crystals. The pH value is in the range of 7.2-7.6. The medium can be adjusted and\/or supplemented according to the performance criteria required.<\/p>\n

Application and Interpretation<\/strong>
\nEach plate is provided with a label including a data matrix code for paperless plate identification. The code consists of a two-dimensional 20-digit serial number, which harbors the following information:
\nDigits 1-3: here code 796 (corresponds to article 146073); digits 4-9: lot number; digits 10-14: batch
\nspecific individual number; digits 15-20: expiration date (YY\/MM\/DD).
\nPlease check each agar plate before using it on sterility and pay attention to aseptic handling in order to avoid false positive results.<\/p>\n

According to the harmonized chapters of EP and USP the absence test for Salmonella is prepared as follows: At first 10 g of the product are cultured within Tryptic Soy Broth (e.g. article number 146334) for 18-24 hours at 30-35 \u00b0C. From this culture 0.1 ml\u00a0 is re transferred into 10 ml Rappaport Vassiliadis Broth (article number 146181) and incubated for 18-24 hours at 30-35 \u00b0C. At least a subculture from Rappaport Vassiliadis Broth is prepared on XLD-Agar and incubated for 18-24 hours at 30-35 \u00b0C. Well developed, red colonies, with or without black centers may indicate the presence of Salmonellae.
\nAccording to the recommendations of EN ISO 6579 for foodstuffs a 1:10 dilution of the sample is prepared within Buffered Peptone Water (e.g. article number 146403) and incubated for 16-20 h at 36-38 \u00b0C.
\nFrom this non-selective pre-enrichment 0.1 ml are transferred into 10 ml Rappaport Vassiliadis Medium (article number 146181) and incubated for 21-27 h at 40.5-42.5 \u00b0C and 1 ml is transferred in 10 ml Tetrathionate Broth (MKTTn, article number 146221) and incubated for 21-27 h at 36-38 \u00b0C. From the selective enrichment cultures each a subculture on XLD Agar is prepared and incubated for 21-27 h at 36-38 \u00b0C. Typical colonies of Salmonella will show a black center and a slightly red colored translucent zone due to the indicator color change. H2S-negative Salmonella (e.g. Salmonella Paratyphi A) will grow pink with a dark pink center. Beside XLD Agar a second selective medium free of choice has to be used.<\/p>\n

The degradation of xylose causes acidification combined with a color change of the medium to yellow. Strains containing lysine-decarboxylase will build up cadaverine (1,5-diaminopentane) from L-lysine which will cause alkalization of the medium indicated by a red violet color of the medium. The activity of lysine-decarboxylase will neutralize acids of xylose degradation and the medium will show a red violet color.<\/p>\n

Lactose and sucrose is added in order to differentiate lysinedecarboxylase-positive coliform bacteria from Salmonella. The formation of acids cannot be neutralized by cadaverine and the medium will be colored yellow. In addition deoxycholate will inhibit the growth of coliform bacteria. Thiosulfate and iron(II) salt will react with hydrogen sulfide to black iron sulphate. Hydrogen sulfide producing bacteria will show a black precipitation center within their colonies.<\/p>\n

Sodium thiosulfate is reduced by Salmonella and other sulfate reducing bacteria to H2S, which will then reduce ferric ammonium citrate to a black colored iron sulfide. This reaction works better under alkaline conditions, wherefore H2S-building bacteria which form acids from lactose and\/or sucrose may not show a strong black center within their colonies (e.g. Citrobacter spp. and Proteus spp.). Salmonella Typhi will show frequently yellow-orange or pink colored colonies.
\nNote: The specificity of the medium is reduced if incubation times exceed 24 hours.<\/p>\n

According to EP and USP suspect colonies have to be identified using suitable methods. The EN ISO 6579 contains detailed information concerning the confirmation of suspect colonies.
\nBesides XLD Agar also the chromogenic Salmonella elective agar according to Rambach (article number 146719) as well as further selective media like BPLS Agar (article number 146046), LEIFSON Agar (article number 102896), Hektoen Enteric Agar (article number 111681), Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Medium (article number 146622) and Salmonella Shigella (SS) Agar (article number 107667) are available.
\nFor biochemical examination of suspect colonies for example Triple Sugar Iron Medium (article number 103915) or Tryptophan Broth (article number 146731) for indole testing are available.<\/p>\n

Storage and Shelf Life<\/strong>
\nThe product can be used for sampling until the expiry date if stored upright, protected from light and
\nproperly sealed at +15 \u00b0C to +25 \u00b0C.
\nCondensation can be prevented by avoiding quick temperature shifts and mechanical stress. The testing procedures as described on the CoA can be started up to the expiry date printed on the
\nlabel.<\/p>\n

Disposal<\/strong>
\nPlease mind the respective regulations for the disposal of used culture medium (e.g. autoclave for 20 min at 121 \u00b0C, disinfect, incinerate etc.).<\/p>\n","protected":false},"excerpt":{"rendered":"

Not In Stock (Back Order Delivery: 4-6 Weeks)<\/b><\/span><\/p>\n","protected":false},"featured_media":482,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[43,45],"product_tag":[],"class_list":{"0":"post-481","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-air-monitoring-media-in-food-beverages-industry","7":"product_cat-li-settle-plates","9":"first","10":"onbackorder","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"yoast_head":"\nXylose Lysine Deoxycholate (XLD) Agar - Ideal Medical Solutions<\/title>\n<meta name=\"description\" content=\"XLD Agar \u2013 LI is a selective culture medium for isolation of Salmonella and Shigella in pharmaceuticals, foodstuff and clinical materials. 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