{"id":4927,"date":"2023-10-13T04:30:17","date_gmt":"2023-10-13T04:30:17","guid":{"rendered":"https:\/\/idealmedical.co.za\/wp\/?post_type=product&p=4927"},"modified":"2025-02-12T14:06:40","modified_gmt":"2025-02-12T14:06:40","slug":"taq-dna-polymerase-with-10x-pcr-reaction-buffer-without-mgcl2-250-units","status":"publish","type":"product","link":"https:\/\/idealmedical.co.za\/wp\/product\/taq-dna-polymerase-with-10x-pcr-reaction-buffer-without-mgcl2-250-units\/","title":{"rendered":"Taq DNA Polymerase with 10\u00d7 PCR reaction buffer without MgCl2 [250 units]"},"content":{"rendered":"
\n

\u00a0from\u00a0Thermus aquaticus<\/i><\/span><\/h3>\n<\/div>\n
Synonyms<\/strong>: Taq polymerase, Taq polymerase enzyme<\/div>\n
\n
\n

General description<\/h3>\n
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium\u00a0Thermus aquaticus<\/i>. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80\u00b0C) has been purified from the extreme thermophile\u00a0Thermus aquaticus<\/i>. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.<\/div>\n<\/div>\n
\n

Application<\/h3>\n
\n

Taq DNA Polymerase from\u00a0Thermus aquaticus\u00a0<\/i>has been used:<\/p>\n

    \n
  • in the process of DNA extraction (during gene amplification and sequencing)<\/li>\n
  • in genotyping<\/li>\n
  • in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8)<\/li>\n
  • for amplification of RNA from primary endothelial cells by conventional PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
    \n

    Biochem\/physiol Actions<\/h3>\n
    Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5\u2032 to 3\u2032 polymerase and exonuclease activities.<\/div>\n<\/div>\n
    \n

    Features and Benefits<\/h3>\n
    \n
      \n
    • MgCl2<\/sub>\u00a0provided in a separate tube to allow MgCl2<\/sub>\u00a0optimization<\/li>\n
    • Can withstand repeated heating to 95 \u00b0C without significant loss of activity<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
      \n

      Packaging<\/h3>\n
      Taq DNA Polymerase with 10\u00d7 reaction buffer without MgCl2<\/sub>. Includes a separate tube of 25\u00a0mM MgCl2<\/sub><\/div>\n
      Taq DNA polymerase comes with the choice of an optimized 10\u00d7 reaction buffer including MgCl2<\/sub>\u00a0(D1806) or a 10\u00d7 reaction buffer without MgCl2<\/sub>\u00a0plus a separate tube of MgCl2<\/sub>\u00a0for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.<\/div>\n<\/div>\n
      \n

      Other Notes<\/h3>\n
      Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium\u00a0Thermus aquaticus<\/i>. The recombinant form of this enzyme is expressed in\u00a0E. coli.<\/i>\u00a0This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5\u2032\u21923\u2032 polymerase and exonuclease activity.<\/div>\n<\/div>\n
      \n

      Unit Definition<\/h3>\n
      One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 \u00b0C.<\/div>\n<\/div>\n
      \n

      Legal Information<\/h3>\n
      Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.<\/div>\n
      \n
      \n

      PROPERTIES<\/h2>\n<\/div>\n
      \n
      \n
      \n
      \n
      \n
      \n
      \n
      \n
      \n
      \n
      \n

      recombinant<\/h3>\n<\/div>\n
      \n

      expressed in\u00a0E. coli<\/i><\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      <\/div>\n
      \n

      \n<\/div>\n<\/div>\n

      \n
      \n

      form<\/h3>\n<\/div>\n
      \n

      liquid<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      usage<\/h3>\n<\/div>\n
      \n

      sufficient for 1500\u00a0reactions<\/span>
      \nsufficient for 250\u00a0reactions<\/span>
      \nsufficient for 50\u00a0reactions<\/span>
      \nsufficient for 5000\u00a0reactions<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      feature<\/h3>\n<\/div>\n
      \n

      dNTPs included: no<\/span>
      \nhotstart: no<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      concentration<\/h3>\n<\/div>\n
      \n

      5\u00a0units\/\u03bcL<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      technique(s)<\/h3>\n<\/div>\n
      \n

      PCR: suitable<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      color<\/h3>\n<\/div>\n
      \n

      colorless<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      input<\/h3>\n<\/div>\n
      \n

      purified DNA<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      suitability<\/h3>\n<\/div>\n
      \n

      suitable for PCR and automated sequencing reactions<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      application(s)<\/h3>\n<\/div>\n
      \n

      agriculture<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      shipped in<\/h3>\n<\/div>\n
      \n

      wet ice<\/span><\/p>\n<\/div>\n<\/div>\n

      \n
      \n

      storage temp.<\/h3>\n<\/div>\n
      \n

      \u221220\u00b0C<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      Not In Stock (Back Order Delivery: 6-8 Weeks)<\/b><\/span><\/p>\n","protected":false},"featured_media":4928,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[115],"product_tag":[],"class_list":{"0":"post-4927","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-molecular-lab-reagents","7":"product_shipping_class-2-8-degrees-celsius","9":"first","10":"onbackorder","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"yoast_head":"\nTaq DNA Polymerase - ideal solutions<\/title>\n<meta name=\"description\" content=\"Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium\u00a0Thermus aquaticus. 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