{"id":5726,"date":"2024-04-20T04:32:24","date_gmt":"2024-04-20T04:32:24","guid":{"rendered":"https:\/\/idealmedical.co.za\/wp\/?post_type=product&p=5726"},"modified":"2025-02-19T03:45:23","modified_gmt":"2025-02-19T03:45:23","slug":"trypan-blue-solution","status":"publish","type":"product","link":"https:\/\/idealmedical.co.za\/wp\/product\/trypan-blue-solution\/","title":{"rendered":"Trypan Blue solution"},"content":{"rendered":"

0.4%, for microscopy<\/span><\/h2>\n
Empirical Formula (Hill Notation):<\/strong> C34<\/sub>H24<\/sub>N6<\/sub>Na4<\/sub>O14<\/sub>S4<\/sub><\/div>\n
\n
\n

General description<\/h3>\n
Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.<\/div>\n<\/div>\n
\n

Application<\/h3>\n
\n

Trypan blue applications include:<\/p>\n

    \n
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.<\/li>\n
  • employed as a collagen stain in a Van Gieson procedure<\/li>\n
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.<\/li>\n
  • used as a fluorescent tracer of cell populations in embryology<\/li>\n
  • used as a tumor promoter modulating permeability of lysosomal membranes<\/li>\n<\/ul>\n

    Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.<\/p>\n<\/div>\n<\/div>\n

    \n

    Biochem\/physiol Actions<\/h3>\n
    Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.<\/div>\n<\/div>\n
    \n

    Physical form<\/h3>\n
    0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic<\/div>\n
    \n
    \n

    PROPERTIES<\/h2>\n<\/div>\n
    \n
    \n
    \n
    \n
    \n
    \n
    \n
    \n
    \n
    \n
    \n

    grade<\/h3>\n<\/div>\n
    \n

    for microscopy<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    Pack Size<\/h3>\n<\/div>\n
    \n

    50mL<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    form<\/h3>\n<\/div>\n
    \n

    liquid<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    shelf life<\/h3>\n<\/div>\n
    \n

    limited shelf life, expiry date on the label<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    concentration<\/h3>\n<\/div>\n
    \n

    0.4%<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    density<\/h3>\n<\/div>\n
    \n

    1.007\u00a0g\/mL\u00a0at 20\u00a0\u00b0C<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    \u03b5max<\/sub><\/h3>\n<\/div>\n
    \n

    1.4 at 603\u00a0nm in methanol<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    application(s)<\/h3>\n<\/div>\n
    \n

    diagnostic assay manufacturing
    \nhematology
    \nhistology<\/span><\/p>\n<\/div>\n<\/div>\n

    \n
    \n

    storage temp.<\/h3>\n<\/div>\n
    \n

    room temp<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

    Not In Stock (Back Order Delivery: No Data From Manufacturer)<\/b><\/span><\/p>\n","protected":false},"featured_media":5727,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[20],"product_tag":[],"class_list":{"0":"post-5726","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-lab-reagent","8":"first","9":"outofstock","10":"shipping-taxable","11":"purchasable","12":"product-type-simple"},"yoast_head":"\nTrypan Blue solution - ideal solutions<\/title>\n<meta name=\"description\" content=\"Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. 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