Description
powder, BioReagent, for molecular biology, suitable for cell culture
Synonyms: Carbamide, Carbonyldiamide
Linear Formula: NH2CONH2
DESCRIPTION
Application
- Urea has been used in protein resuspension buffer and to denature proteins.
- It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
- It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
PROPERTIES
grade
for molecular biology
Pack Size
500g
product line
BioReagent
Assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
132-135 °C (lit.)
solubility
H2O: soluble 480 mg/mL, clear, colorless (8M)
density
1.335 g/mL at 25 °C (lit.)
anion traces
chloride (Cl–): ≤5 ppm
cation traces
Cu: ≤1 ppm
Fe: ≤5 ppm
Pb: ≤2 ppm
absorption
≤0.06 at 260 at 6 M
≤0.06 at 280 at 6 M
foreign activity
DNase, NICKase, RNase and protease, none detected
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