Description
Application
Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 – 2 μg/ml of DNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 – 2 μg/ml of DNA.
PROPERTIES
Pack Size
10mL
Assay
≥95.0% (HPCE)
form
liquid
solubility
DMSO: soluble
fluorescence
λex 520 nm; λem 554 nm in TE buffer; DNA
storage temp.
2-8°C
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Greener alternative to Ethidium bromide.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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