1 kb DNA Ladder

R5,011.70

Items in stock, allow 5-7 working days delivery period within South Africa and 7-10 working days for orders within Southern Africa

1 in stock (can be backordered)

SKU: ZA.003.397 Category:

Description

for DNA electrophoresis

Synonyms: 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Components

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

PROPERTIES

Pack Size

1 vial

form

liquid

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

SYBR is a registered trademark of Life Technologies

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