2-Mercaptoethanol

R1,319.59

Not In Stock (Available on Back-Order); Delivery: 2-4 Weeks

Available on backorder

Description

BioUltra, for molecular biology, ≥99.0% (GC)

Synonyms: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
Linear Formula: HSCH2CH2OH

Application

2-Mercaptoethanol has been used

  • in the protein extraction buffer prepared for leaf samples.
  • as a supplement in cell culture media.
  • in the procedure of RNA extraction.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

PROPERTIES

grade

for molecular biology

Pack Size

25mL

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioUltra

Assay

≥99.0% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

RNA extraction: suitable
protein extraction: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.500 (lit.)
n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.114 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.05 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

OCCS

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3

storage temp.

2-8°C

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