ALT/GPT Clinical Chemistry reagent, Elitech

Description

INTENDED USE

ELITech Clinical Systems ALT/GPT 4+1 SL is intended for the quantitative in vitro diagnostic determination of Alanine amino transferase (ALT) in human serum and plasma.

CLINICAL SIGNIFICANCE

Alanine aminotransferase (ALT), also known as gluta- mate pyruvate transaminase (GPT), is a transaminase. ALT catalyses the transfer of the amino group of L-alanine to α-ketoglutarate to give L-glutamate. The highest levels are found in the liver and the kidneys, and in smaller amounts in heart and skeletal muscle. ALT concentration is increased when hepatic cells are damaged (liver cell necrosis or injury of any cause). Indeed, viral and toxic hepatitis induce a marked eleva- tion of ALT activity in serum. Intake of alcohol, delirium tremens, and administration of various drug induce slight or moderate elevation of ALT. ALT concentration in serum is also slightly increased in various conditions such as: muscular dystrophy, hemolytic  disease, myocardial infarction…

ALT is more liver specific than AST (Aspartate aminotransferase). Measurement of both AST and ALT has some value in distinguishing hepatitis from other parenchymal lesions.

ALT serum level can be decreased in case of vitamin B6 deficiency.

METHOD

IFCC method without pyridoxal phosphate (P-5’-P). Kinetic. UV.

PRINCIPLE 

Kinetic determination of alanine aminotransferase (ALT) activity:

REAGENTS COMPOSITION

Reagent 1 : R1

Tris buffer, pH 7.50 (30°C) 125 mmol/L L-Alanine 680 mmol/L

LDH ≥   2 000 U/L

Sodium azide < 0.1 %

Reagent 2 : R2

Ketoglutarate 97 mmol/L

NADH 1,1 mmol/L

Sodium azide < 0.1 %

MATERIALS REQUIRED BUT NOT PROVIDED

• CALI-0550 ELICAL 2 4 x 3 mL
• CONT-0060 ELITROL I 10 x 5 mL
• CONT-0160 ELITROL II  10 x 5 mL
• General Laboratory equipment.