AST/GOT 4+1 Clinical Chemistry reagent, Elitech

Description

INTENDED USE

ELITech Clinical Systems AST/GOT 4+1 SL is intended for the quantitative in vitro diagnostic determination of Aspartate aminotransferase (AST) in human serum and plasma.

CLINICAL SIGNIFICANCE (1-4)

Aspartate aminotransferase (AST), also known as glutamate oxaloacetate transaminase (GOT), is a transaminase. AST catalyses the transfer of the aminogroup of L-aspartate to α-ketoglutarate to give L-glutamate. AST is widely distributed in the body, but the highest levels are found in heart, liver, skeletal muscles and kidneys.

Damage to cells of these tissues induces AST increase in serum. In case of fulminant forms of hepatitis, especially viral hepatitis the enzyme level is markedly elevated. In case of myocardial infarction, AST activity increases and reaches a peak after 18-24 hours. The activity falls back to normal after 4-5 days, provided no new infarct has occurred.

The following pathological states are examples of disorders also resulting in an increase of enzyme activity : liver cell necrosis or injury of any cause (for example intake of alcohol, delirium tremens, and administration of drug induce moderate AST elevation), alcoholic hepatitis, muscular dystrophy and gangrene, infectious mononucleosis, acute pancreatitis, cardiac afflictions such as myocarditis or pericarditis, pulmonary emboli…

AST serum level can be decreased in case of vitamin B6 deficiency.

METHOD (5)

IFCC method without pyridoxal phosphate (P-5’-P). Kinetic. UV.

PRINCIPLE (5)

Kinetic determination of aspartate aminotransferase (AST) activity :

• REAGENTS COMPOSITION

Reagent 1 : R1

Tris buffer, pH 7.80 (30°C) 100 mmol/L L-Aspartate 330 mmol/L

LDH ≥   2 000 U/L

MDH ≥   1 000 U/L

Sodium azide < 0.1 %

Reagent 2 : R2

α-Ketoglutarate 78 mmol/L

NADH 1.1 mmol/L

Sodium azide < 0.1 %

MATERIALS REQUIRED BUT NOT PROVIDED

• CALI-0550 ELICAL 2 4 x 3 mL
• CONT-0060 ELITROL I 10 x 5 mL
• CONT-0160 ELITROL II  10 x 5 mL
• Normal saline solution (NaCl 9 g/L).
• General Laboratory equipment.
• Do not use materials that are not required as indicated above.