Description
Synonym(s): Pyoctaninum aureum, 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2
Empirical Formula (Hill Notation): C17H21N3 · HCl
DESCRIPTION
Application
Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.
Packaging
Biochem/physical Actions
Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.
Suitability
Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.
PROPERTIES
grade: certified by the Biological Stain Commission
form: powder or chunks
composition: Dye content, 85%
mp: >250 °C (dec.) (lit.)
solubility: 95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow
λmax
370 nm
434 nm (2nd)
ε (extinction coefficient)
≥12000 at 249-255 nm in water at 0.005 g/L
≥15000 at 367-373 nm in water at 0.005 g/L
≥36000 at 429-435 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
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