Biuret reagent

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.

Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.