Description
for 0.1-1.4 mg/ml protein
Synonyms: Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent
General description
Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.
Application
Bradford Reagent has been used to determine total protein concentration.
Features and Benefits
- The reagent is ready to use. No mixing or dilution required.
- Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
- Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
- Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
- Can be used in microwell plate assays.
- Inexpensive assay.
PROPERTIES
Pack Size
500mL
form
solution
storage temp.
2-8°C
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