Description
Application
A highly selective medium for the isolation and identification of Salmonella except S. typhosa or Shigella species.
Preparation Note
Suspend 52 g in 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. Do not autoclave! Cool to 50°C, mix well and pour plates.
Other Notes
Salmonella isolation in 9 European laboratories using a standardized technique; Variation in plating efficiency of Salmonellae in eight lots of Brilliant Green Agar.
PROPERTIES
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 12.0 g/L
bacteriological peptone, 10.0 g/L
brilliant green, 0.0047 g/L
disodium hydrogen phosphate, 1.0 g/L
lactose, 10.0 g/L
meat extract, 5.0 g/L
phenol red, 0.09 g/L
sodium dihydrogen phosphate, 0.6 g/L
sucrose, 10.0 g/L
yeast extract, 3.0 g/L
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
final pH
6.9±0.2 (25 °C)
application(s)
environmental
food and beverages
water monitoring
microbiology
suitability
selective and differential for Salmonella spp.
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