Plate Count Agar Contact 55 mm for the determination of total count of aerobic bacteria [Pack of 20]

Description

Packaging: 55 mm contact plate (20 plates/box)
Synonym: Tryptone Glucose Yeast Agar, Casein-Peptone Dextrose Yeast Agar, Standard Methods Agar, PCA

Application: Environmental monitoring determination of total count of aerobic bacteria, Room temperature storage

Plate Count Agar is suitable for the determination of the total count of aerobic bacteria from milk, dairy products, water, and other sample materials.
The formulation of the basic medium complies with ISO 4833 and FDA-BAM.
Plate Count Agar is also called Tryptone Glucose Yeast Agar or Casein-Peptone Dextrose Yeast Agar.

Mode of Action
This medium does not contain any inhibitors or indicators and it is relatively rich in its nutrients. The enzymatic digest of casein (tryptone) is a nitrogen source containing a high level of free amino acids and yeast extract primarily supplies the B-complex vitamins. Glucose provides an energy source for the growth of bacteria whilst agar is the solidifying agent.

Typical Composition
Casein Peptone 5 g/l
Yeast Extract 2.5 g/l
Glucose 1 g/l
Agar 9-18 g/l
The appearance of the medium is clear and slightly yellowish. The pH value is in the range of 6.8-
7.2. The medium can be adjusted and/or supplemented according to the performance criteria
required.
Application and Interpretation
Each plate is provided with a label including a data matrix code for paperless plate identification.
The code consists of a two-dimensional 20-digit serial number, which harbors the following
information:
digits 1-3: here code 865 (corresponds to article 146154); digits 4-9: lot number; digits 10-
14: batch specific individual number; digits 15-20: expiration date (YY/MM/DD).
Please check each agar plate before using it on sterility and pay attention to aseptic handling in
order to avoid false positive results.
The recovery rate of all bacteria tested is given at 70 %. The culture medium is inoculated with the
original sample material and/or in appropriate dilutions material using a sterilized spreader rod.
For the determination of the total count of aerobic bacteria using the pour plate method, the
agar has to be liquefied at 95 °C in a water bath (tubes for 10 minutes, 200 ml bottles for 30 minutes,
400 ml bottles for 60 minutes) and afterwards cooled down in a second water bath with 45 °C
Lit. No. TN2400EN00
(cooling times correlate with the respective heating times). The liquefied agar is then poured over
the samples in the petri dishes, which are then swirled to mix the contents.
The culture medium is incubated aerobically for 24-48 hours at 29-31 °C.
Storage and Shelf Life
The product can be used for sampling until the expiry date if stored upright, protected from light
and properly sealed at +2 °C to +25 °C.
Condensation can be prevented by avoiding quick temperature shifts and mechanical stress.
The testing procedures as described on the CoA can be started up to the expiry date printed on the
label.
Disposal
Please mind the respective regulations for the disposal of used culture medium (e.g. autoclave for 20
min at 121 °C, disinfect, incinerate etc.).