Description
Synonyms: EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
Linear Formula: [-CH2OCH2CH2N(CH2CO2H)2]2
Application
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used:
- in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
- as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
- as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis
A chelating agent useful for the determination of calcium in the presence of magnesium.
Biochem/physiol Actions
Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca2+) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca2+ channels. This metal chelator can inhibit the activity of pseudolysin.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.
PROPERTIES
grade
for molecular biology
Assay
≥97.0%
form
powder
reaction suitability
reagent type: chelator
loss
≤1.0% loss on drying
mp
241 °C (dec.) (lit.)
foreign activity
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
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