Evans Blue Dye content ≥75 %, Powder

R1,991.28

Not In Stock (Back Order Delivery: 6-8 Weeks)

SKU: ZA.003.062 Category:

Description

Synonym: Direct Blue 53

Empirical Formula (Hill Notation): C34H24N6Na4O14S4

General description

Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.

Application

Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

PROPERTIES

product name

Evans Blue, Dye content ≥75 %

form

powder

composition

Dye content, ≥75%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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