HbA1c Clinical Chemistry Reagent, Elitech: 1 x 32 ml, Generic [89 Tests]

Description

INTENDED USE

This reagent is intended for in vitro quantitative determination of % HbA1c in human blood- Latex enhanced lmmunoturbidimetry.

– Ready to use stable liquid reagents

– Multipoint calibration

– Direct result (% HbA1c) from analyzer

– No need to measure total Hb.

CLINICAL SIGNIFICANCE

HbAlc is a glycated form of hemoglobin formed by the attachment of glucose residues in the blood to the hemoglobin molecules. In the diabetic population where blood glucose levels are abnormally elevated, the level of HbA1c also increases. The level of HbAlc is proportional to the level of glucose in the blood and has been widely
accepted as an indicator of the mean blood glucose concentration in the preceding 6-8 weeks. It is therefore a long-term indicator of diabetic control. For routine use HbAlc levels should be monitored
every 3-4 months.

PRINCIPLE 

The whole blood is lysed using haemolysing reagent. The lysed whole blood containing HbAlc along with other haemoglobins compete to adsorb to the unsensitised latex particles in the Rl. A mouse antihuman HbA1c monoclonal antibody is added into the reaction that specifically binds to the human HbAlc molecules to form latex HbAlc-  mouse  antihuman  HbA1c  antibody  complex. Another antibody, goat anti mouse polyclonal antibody that react with the formed complex to give agglutination. The amount of agglutination is proportional to the amount of HbAlc adsorbed on to the surface of latex particles, It is measured at 670 nm which is used to calculate the HbAlc % from a calibration curve.