Description
General description
Capacity: The High Pure SpinFilter Tubes hold up to 700 μl sample volume.
Volume: 100 μl
Typical samples include:
- Products from amplification or cDNA synthesis reactions
- Enzymatically treated DNA
- DNA from agarose slices
- Dilute nucleic acid solutions
- RNA from transcription reactions
Application
- Labeling
- Sequencing
- Cloning
- Restriction enzyme digests
- Alkaline phosphatase treatment
- Kinase reactions
The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications.
The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 μl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR.
Features and Benefits
- Quickly purifies multiple PCR products
in <10 minutes, and purify DNA from agarose gel slices in <20 minutes.
- Efficiently recover DNA fragments
>100 bp in length, and concentrate dilute nucleic acid solutions.
- Minimize DNA loss
with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
- Eliminate the use of hazardous organic compounds
such as cesium chloride, phenol, chloroform, and ethidium bromide
Components
- Binding Buffer
- Wash Buffer
- Elution Buffer
- High Pure Spin Filter Tubes (containing glass fiber fleece)
- Collection Tubes
Quality
Preparation Note
PROPERTIES
usage
sufficient for 50 purifications
manufacturer/tradename
Roche
technique(s)
DNA purification: suitable
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