Description
General description
A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.
Application
Hydrogen peroxide solution has been used:
- to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
- for the induction of apoptosis in cultured human microvascular endothelial cells
- as a substrate for superoxide dismutase
PROPERTIES
Agency
according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017
vapor pressure
23.3 mmHg ( 30 °C)
assay
2.8-3.2% (redox titration)
form
solution
shelf life
limited shelf life, expiry date on the label
concentration
1-5%
technique(s)
microbe id | specific enzyme detection: suitable
mp
0.0 °C
density
1.000 g/cm3
application(s)
clinical testing
environmental
food and beverages
veterinary
microbiology
suitability
Pneumococcus spp.
Staphylococcus spp.
bacteria
SMILES string
OO
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