LB broth for microbiology (MILLER), 250g

R1,440.29

Items in stock, allow 5-7 working days delivery period within South Africa and 7-10 working days for orders within Southern Africa

1 in stock (can be backordered)

SKU: ZA.002.928 Category:

Description

Synonyms: Luria Bertani Broth, Luria Bertani Medium

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coliPseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

PROPERTIES

grade

for molecular biology

Pack Size

250g

description

Miller′s Modification, 10 g/L NaCl

sterility

non-sterile

form

powder

composition

NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

storage temp.

room temp

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