Description
Synonym: M-S Agar
DESCRIPTION
Application
Mitis Salivarius Agar is recommended for the isolation from mixed cultures of Streptococci especially Streptococcus mitis, Streptococcus salivarius, Enterococcus faecalis showing α and γ haemolytic reactions on Blood Agar.
Preparation Note
Suspend 90 g in 1000 ml distilled water. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and add 1 ml of sterile 1 % potassium tellurite solution . DO NOT REHEAT the medium after the addition of tellurite solution.
PROPERTIES
sterility
non-sterile
Pack Size
500g
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15.0 g/L
casein enzymatic hydrolysate, 15.0 g/L
crystal violet, 0.0008 g/L
dextrose, 1.0 g/L
dipotassium phosphate, 4.0 g/L
peptic digest of animal tissue, 5.0 g/L
sucrose, 50.0 g/L
trypan blue, 0.075 g/L
manufacturer/tradename
NutriSelect® Plus
final pH
7.0±0.2 (25 °C)
application(s)
clinical testing
food and beverages
veterinary
microbiology
suitability
selective and differential for Enterococcus spp.
selective and differential for Streptococcus spp.
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