Description
Application
Used for the identification of enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.
Components
Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 3.00
L-Ornithine hydrochloride, 5.00
Dextrose, 1.00
Bromocresol purple, 0.02
Agar, 2.00
Preparation Note
Suspend 31 g of MIO Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense 5 ml into each test tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the tubes in an upright position.
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