mTSB Broth with Novobiocin, 500g

Description

General description
mTSB Broth with Novobiocin has been used for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption (according to ISO 16654). The medium is used for the isolation and identification of Escherichia coli O157:H7 from meat according to the USDA-FSIS method. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli. mTSB is made selective for E. coli O157:H7 by the addition of bile salts in the media along with being supplemented with Novobiocin.

Application
mTSB Broth with Novobiocin has been used for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods. The medium is used for the isolation and identification of Escherichia coli 0157:H7 from meat acc. the USDA-FSIS method.

Preparation Note
Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

PROPERTIES

sterility
non-sterile

form
powder

shelf life
limited, expiry date on the label

composition
bile salts No. 3, 1.5 g/L
di-potassium hydrogen phosphate, 4.0 g/L
D(+)-glucose, 2.5 g/L
novobiocin, 0.02 g/L
peptone from casein, 17.0 g/L
peptone from soymeal, 3.0 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename
NutriSelect® Plus

technique(s)
microbiological culture: suitable

final pH
7.3±0.2 (25 °C)

application(s)
agriculture
food and beverages
pathogen testing
microbiology