Description
Synonyms: 1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate
Empirical Formula (Hill Notation): C9H6O4
Molecular Weight: 178.14
General description
Ninhydrin, a tricyclic 1,2,3-trione, is a useful organic building block. It is an amino acid reagent, which reveals latent fingerprints on porous surfaces like paper, cardboard and raw wood.
Application
Ninhydrin (Triketohydrindene hydrate) may be used as a reagent for the quantification of amino acids and peptides by photometric method and colorimetric method.
It may be used to prepare the ninhydrin reagent, by mixing ninhydrin and hydrindantin (reduced form of ninhydrin) in dimethyl sulfoxide. This reagent is used in manual ninhydrin method for the quantitative determination of amino acids.
Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines. Reaction with sarcosine or proline gives azomethine ylides.
Used for the detection of free amino groups in amino acids, peptides and proteins.
Product Name
Ninhydrin, ACS reagent
grade
ACS reagent
description
identification and melting point passes test
form
solid
color
white to brownish white, crystals
mp
250 °C (dec.) (lit.)
solubility
H2O: passes test
suitability
passes test for sensitivity to amino acids
functional group
hydroxyl
ketone
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