ReadyMix™ Taq PCR Reaction Mix [100 reactions]

ReadyMix^™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

ReadyMix^™ Taq PCR Reaction Mix has been used:

• in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers
• in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis
• in the molecular identification of yeasts
• for routine PCR amplifications

• Amplifies targets up to 7 kb in length

• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

• ReadyMix format reduces setup time.

• Reduced risk of contamination from multiple pipet steps.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL

Supplied with a vial of PCR grade water for dilution.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.