ReadyPlate™ CT DG 18 (Dichloran Glycerol Chloramphenicol) Agar [Pack of 20]

Description

For enumeration of yeast and moulds of products with less water activity. This culture medium complies with the specifications given by ISO 21527-2

Mode of Action
By reducing the water activity from approx. 0.99 to 0.95 with 18 % (w/w) glycerol and addition of chloramphenicol growth of bacteria is prevented. The inclusion of dichloran serves to inhibit the rapid spreading of mucoraceous fungi and restricts colony sizes of other genera, easing the colony count.

Typical Composition

Casein Enzymatic Digest     5 g/l
Glucose      10 g/l
KH2PO4     1 g/l
MgSO4       0.5 g/l
Dichloran    0.002 g/l
Chloramphenicol   0.1 g/l
Agar-Agar             15 g/l
Glycerol, anhydrous    220 g/l
Water                         1000 ml/l
pH at 25 °C                5.6 ± 0.2

Application and Interpretation
Each plate is provided with a label including a data matrix code for paperless plate identification. The code consists of a two-dimensional 20-digit serial number, which harbors the following information:
Digits 1-3: here code 870 (corresponds to article 146266); digits 4-9: lot number; digits 10-14: batch
specific individual number; digits 15-20: expiration date (YY/MM/DD).

We provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.
Please check each agar plate on sterility before using it and pay attention to aseptic handling in order
to avoid false positive results.
The culture medium is incubated aerobically at 24-26 °C for 5 to 7 days.
Colonies or propagules are counted and if required doubtful colonies can be examined with a (stereo-) microscope to distinguish yeast colonies from possible bacterial colonies.
The number of yeast and molds per g or ml may be counted and calculated separately if necessary.

Storage and Shelf Life
The product can be used for tests until the expiry date if stored upright, protected from light and properly sealed at +15 °C to +25 °C. Shelf life of 180 days.
Condensation can be prevented by avoiding quick temperature shifts and mechanical stress.
The testing procedures as described on the CoA can be started up to the expiry date printed on the label.

Disposal
Please mind the respective regulations for the disposal of used culture medium (e.g. autoclave for 20
min at 121 °C, disinfect, incinerate etc.).