Sabouraud Dextrose Agar settle plates– Long incubation [Pack of 20]

Description

Ten settle plates each with a diameter of 90 mm are single-bagged in transparent, hydrogen peroxide
impermeable sleeves (non-irradiated). The sleeves consist of polypropylene with a barrier of PE-EVOHPE.
This medium complies with the recommendations of the harmonized methods of EP, USP, JP for Microbial Examination of Non-sterile Products: Microbial Enumeration Test and Tests for Specified Microorganisms.
Sabouraud Dextrose (4%) Agar – LI is available with the identical media formulation with and without
the addition of Chloramphenicol:

• Sabouraud Dextrose (4%) Agar – LI + Chloramphenicol (article number 146003; DMC: 788): 90
mm settle plates, single-bagged; intended for determination of the total count of yeasts and
molds. The plate design allows aerobic incubation only.

Mode of Action

Sabouraud Dextrose Agar (SDA) is a complex medium for cultivation and isolation of yeasts and molds
as well as the absence test for Candida albicans. The high concentration of Dextrose in addition with
the low pH promotes the growth, the formation of spores (conidia and sporangia) as well as the formation
of pigments of yeasts and molds. On the other side, the growth of bacteria is inhibited.

Typical Composition
Casein Peptone           5 g/l
Meat Peptone              5 g/l
Dextrose                    40 g/l
Agar                          15 g/l

The appearance of the medium is clear and yellowish. The pH value is in the range of 5.4-5.8. The medium can be adjusted and/or supplemented according to the performance criteria required.

We provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.

Application and Interpretation
Each plate is provided with a label including a data matrix code for paperless plate identification. The
code consists of a two-dimensional 20-digit serial number, which harbors the following information:
Digits 1-3: here code 789 (corresponds to article 146028); digits 4-9: lot number; digits 10-14: batch
specific individual number; digits 15-20: expiration date (YY/MM/DD).

Please check each agar plate before using it on sterility and pay attention to aseptic handling in order
to avoid false positive results.
For determination of the TYMC (total yeasts and mold count) using the membrane filtration method the
following procedure is described in the harmonized chapters of EP, JP and USP:
The product is dissolved or diluted in buffered NaCl Solution (article number 146648), Phosphate Buffer
pH 7.2 or Tryptic Soy Broth (article number 146317).
A suitable amount of the diluted sample (preferably 1 g or 1 ml of the original sample) is transferred onto
the membrane filter and filtered immediately. Afterwards the filter is rinsed with an appropriate volume
of diluent.

The filter is transferred onto a Sabouraud Dextrose Agar and incubated at 20-25 °C for 5-7 days.
For the detection of Candida albicans in non-sterile products not less than 1 g of the sample is pre-enriched in Sabouraud Dextrose Broth (article number 146366) at 30-35 °C for 3-5 days.

Afterwards a subculture on Sabouraud Dextrose Agar is prepared and incubated at 30-35 °C for 24-48 hours. White
colonies may indicate the presence of C. albicans.

Storage and Shelf Life
The product can be used for sampling until the expiry date if stored upright, protected from light and properly sealed at +15 °C to +25 °C.
Condensation can be prevented by avoiding quick temperature shifts and mechanical stress. The testing procedures as described on the CoA can be started up to the expiry date printed on the label.

Disposal
Please mind the respective regulations for the disposal of used culture medium (e.g. autoclave for 20 min at 121 °C, disinfect, incinerate etc.).