Sodium dodecyl sulfate

Sodium dodecyl sulfate (SDS) is an ionic detergent, which contains a polar sulfate head group and a hydrophobic 12-carbon chain.

Sodium dodecyl sulfate (SDS) has been used:

• as a negatively charged surfactant to study its effects on filtration flux and membrane fouling during the microfiltration of the surfactant-stabilized oil emulsion
• as a component of plant extraction buffer, and transferring buffer for western blot
• as a component of 5× Laemmli sample buffer (5× LB) and 10× SDS running buffer for sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot

Anionic detergent

Sodium dodecyl sulfate (SDS) effectively binds to the positively charged and the hydrophobic residues of proteins via sulfate groups and alkyl chains. It functions as a vital detergent in the domain of structural biology and protein folding/unfolding studies. SDS can stimulate the creation of α-helices in a protein structure. It has the ability to promote cooperative unfolding in several proteins including bovine serum albumin (BSA), β-lactoglobulin, α-lactalbumin, and hen egg-white lysozyme structures at elevated concentrations.