Tris base

R1,822.77

Not In Stock (Back Order Delivery: 4-6 Weeks)

SKU: ZA.002.202 Category:

Description

=99.9% (titration), abs., 100 mg/mL, 300 nm ≤0.015

Synonyms: Trizma® base, amino-2-(hydroxymethyl)-1,3-propanediol, 2-, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula: NH2C(CH2OH)3

DESCRIPTION

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

  • As a component of lysis buffer for cell disruption
  • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Quality

Contaminants: <0.001% PK and aldolase, each, <0.01% GOT, GPT, MDH and myokinase, each

PROPERTIES

description

aminopeptidase substrate

Assay

99.9% (titration)

packaging

pkg of 1 kg 

manufacturer/tradename

Roche

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

absorption

≤0.015 at 300 nm at 100 mg/mL

storage temp.

20-25°C

Other Notes

For life science research only. Not for use in diagnostic procedures.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Additional information

Pack Size

500g, 1kg

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