Urease from Canavalia ensiformis (Jack bean)

R8,036.22

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SKU: ZA.003.655 Category:

Description

powder, ~1 U/mg

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C.  Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes. It may be useful for the detection of pathogens as well as heavy-metal ions.
Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.

PROPERTIES

form

powder

Pack Size

25g

specific activity

~1 U/mg

mol wt

Mr ~480000

storage temp.

−20°C

Other Notes

Application in (selective) hydrolysis/condensations; structure.

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