Description
Tryptic Soy Broth (TSB)
CASO Broth (Casein-peptone Soymeal-peptone Broth)
Application
A versatile liquid medium suitable for general laboratory use. Due to its high nutrient value, it will produce luxuriant growth of many fastidious organisms without the addition of serum.
Typical Composition (g/litre)
Peptone from casein 17.0;
peptone from soymeal 3.0;
D(+)glucose 2.5;
sodium chloride 5.0;
di-potassium hydrogen
phosphate 2.5.
Preparation
Suspend 30 g/litre, autoclave (15 min at 121 °C).
pH: 7.3 ± 0.2 at 25 °C.
After preparation both media are clear and yellowish-brown.
Experimental Procedure and Evaluation
Depend on the purpose for which the media are used.
Incubation: 24 hours at 35 °C aerobically, for up to 7 days for the
sterility-test at room-temperature.
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