Lactose Broth, 500g

$63.46

Inhibitor-free culture medium used as a preliminary test for coliform bacteria, especially E. coli

59 in stock

SKU: ZA.002.008 Category:

Description

The composition of this medium complies with the recommendations of the American Public Health Association for
the examination of water (1998) and foodstuffs (1992), and with the recommendations of the United States Pharmacopeia XXVI (2003) and the European Pharmacopeia II for the examination of pharmaceutical products and raw materials.


Mode of Action
Lactose utilization is indicated by gas production. The gas liberated is collected in DURHAM tubes.


Typical Composition (g/litre)
Peptone 5.0; meat (beef) extract 3.0; lactose 5.0.


Preparation
Suspend 13 g or more/litre (see Table), dispense into test tubes fitted with DURHAM tubes, autoclave (15 min at 121 °C).

pH: 6.9 ± 0.2 at 25 °C.
The prepared broth is clear and yellowish.


Experimental Procedure and Evaluation
Mix 1, 10 or 100 ml samples with the specified volumes of lactose broth. The initial concentaton of the lactose broth must be increased so that the final concentration of the components is maintained at a constant level (13 g/l). See table

Inoculum
(ml)
Amount
of
Medium
in Tube
ml
Volume
of
Medium
+
Inoculum
ml
Dehydra
ted
Lactose
Broth
Required
g/L
Broth
concen
tration
1 10 or more 11 or more 13 1-fold
10 10 20 26 2-fold
10 20 30 19.5 1.5-fold
100 20 120 78 6-fold
100 50 150 39 3-fold

Incubation: 24-48 hours at 35 °C aerobically.
Check the DURHAM tubes for gas production.

Additional information

Weight 0.5 kg

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