Description
Dye content 75 %
Synonyms: 3,6-Bis(dimethylamino)acridine, Solvent Orange 15
Empirical Formula (Hill Notation): C17H19N3
Molecular Weight: 265.35
Colour Index Number: 46005
General description
Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.
Application
Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.
PROPERTIES
form
powder
composition
Dye content, 75%
technique(s)
microbe id | staining: suitable
mp
165 °C (dec.) (lit.)
solubility
1 M HCl: 1 mg/mL, clear
λmax
488 nm
ε (extinction coefficient)
≥14000 at 227-233 nm
≥20000 at 289-295 nm
≥35000 at 486-492 nm
≥40000 at 266-272 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
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