Description
General description
rapid identification kit for E. coli
BACTIDENT™ E. coli test strips are impregnated with 4-methylumbelliferyl-β-D-glucuronide (MUG), which is cleaved to form 4-methylumbelliferone in the presence of β-D-Glucuronidase. The light blue fluorescence upon excitation with long-wavelength UV light, indicates the presence of the enzyme. Tryptophanase activity is confirmed if the bacterial suspension turns red upon addition of KOVACS′ reagent due to indole formation.
Application
BACTIDENT™ E. coli is used for the bioburden testing in drinking water. This test allows for the rapid detection of coliform organisms (E. coli) by testing the bacterial culture for the presence of enzymes β-D-Glucuronidase and Tryptophanase.
PROPERTIES
feature
ready-to-use
manufacturer/tradename
Bactident™
technique(s)
microbe id | specific enzyme detection: suitable
input
water
sample(s) (environmental)
application(s)
detection
environmental
food and beverages
storage temp.
2-8°C
suitability
Citrobacter spp.
Escherichia coli
Proteus spp.
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