Description
Synonyms: Bromophenol blue, 3,3′,5,5′-Tetrabromophenolsulfonphthalein, BPB
Empirical Formula (Hill Notation): C19H10Br4O5S
DESCRIPTION
General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.
Transition range: pH 3.0 – pH 4.6greenish yellow – blue violet
Appearance of solution: passes test
Loss on drying (110 °C): ≤ 1
Transition range (according to ACS): passes test
Sensitivity test: passes test
Appearance of solution: passes test
Loss on drying (110 °C): ≤ 1
Transition range (according to ACS): passes test
Sensitivity test: passes test
PROPERTIES
grade
ACS reagent
Agency
reag. Ph. Eur.
form
solid
loss
≤1% loss on drying, 110°C
visual transition interval
3.0-4.6, greenish yellow to blue-violet
mp
270-273 °C (decomposition)
bulk density
730 kg/m3
storage temp.
2-30°C
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