Brucella Agar Base 500g

Description

This culture medium can be utilized as it is or as a base for the preparation of special culture media. It complies with the recommendations of WHO (1953) and HAUSLER and KOONITZ in Diagnostic Procedures (1970).

For the isolation and cultivation of Brucella species, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin.

Mode of Action
KUZDAS and MORSE (1953), RENOUX (1954) and WEED (1957) demonstrated that, in the case of heavily contaminated sample material, the growth of accompanying microbial flora can be suppressed by addition of bacitracin, polymyxin, cycloheximide and possibly ethyl violet. Circulin, which also has been recommended originally, is no longer used (ALTON and JONES 1967).

The various Brucella species can be differentiated by exploiting the fact that they show different sensitivities towards the dyes thionine and fuchsin. Differential culture media can be prepared by adding these two compounds to Brucella agar.

Typical Composition (g/litre)
Peptone from meat 10.0; peptone from casein 10.0; yeast extract 2.0; D(+)glucose 1.0; sodium chloride 5.0; agar-agar 13.0.

Preparation
Suspend 41 g/litre, autoclave (15 min at 121°C), pour plates. pH: 7.0 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.

Preparation of Brucella differential agar: Sterilize Brucella agar, cool, adjust pH to 6.7 ± 0.1. To 1 litre add 1 ml (1:100,000), 2 ml (= 1:50,000) or 4 (=1:25,000) of an aqueous 1 % thionine or basic fuchsin solution, mix. The solutions should first be heated for 20minutes in a boiling water bath.

Preparation of Brucella selective agar: Sterilize the Brucella agar, cool to 45-50 °C, add filter-sterilized solutions of the following compounds: