Description
Application
A selective medium recommended for the isolation of enteric pathogens particularly Salmonella and Shigella species.
Components
Components (g/L)
Heart infusion solids 10.00
Proteose peptone 10.00
Lactose 10.00
Sodium citrate 20.00
Ferric ammonim citrate 2.00
Sodium deoxycholate 5.00
Neutral red 0.02
Agar 13.50
Heart infusion solids 10.00
Proteose peptone 10.00
Lactose 10.00
Sodium citrate 20.00
Ferric ammonim citrate 2.00
Sodium deoxycholate 5.00
Neutral red 0.02
Agar 13.50
Preparation Note
Suspend 70.52 g of Deoxycholate Citrate Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Do not autoclave. Avoid excessive heating as it is detrimental to the medium.
PROPERTIES
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
pH
7.5±0.2 (25 °C)
application(s)
environmental
food and beverages
microbiology
suitability
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
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