Description
Application
Used as a confirmative medium for detecting and confirming Enterococci and as an indicator of faecal pollution in water and other specimens.
Preparation Note
Suspend 35.8 g in 1 litre distilled water. Heat, if necessary to dissolve the medium completely. Dispense in tubes in 10 ml amounts and sterilize by autoclaving at 121°C for 15 minutes.
Properties
form
powder
shelf life
limited shelf life, expiry date on the label
Composition
casein enzymic hydrolysate, 20 g/L
dextrose, 5 g/L
dipotassium phosphate, 2.7 g/L
ethyl violet, 0.00083 g/L
monopotassium phosphate, 2.7 g/L
sodium azide, 0.4 g/L
sodium chloride, 5 g/L
final pH
7.0±0.2 (25 °C)
Application(s)
environmental
food and beverages
water monitoring
microbiology
suitability
selective for Enterococcus spp.
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