Glycine for electrophoresis, ≥99% [500g]

R2,212.18 R3,582.55Price range: R2,212.18 through R3,582.55

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Description

Synonyms: Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula: NH2CH2COOH

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Application

  • Glycine has been used for the preparation of gelatin composites scaffold.
  • It has been used in the elution buffer to extract protein using HiTrap protein G column.
  • It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

PROPERTIES

Assay

≥99%

form

powder

technique(s)

electrophoresis: suitable
western blot: suitable

impurities

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubility

H2O: 200 mg/mL, clear, colorless to faintly yellow

anion traces

chloride (Cl): ≤70 ppm

cation traces

heavy metals (as Pb): ≤20 ppm

absorption

≤0.15 at 280 at 1 M

Additional information

Pack Size

500g, 1kg

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