Description
Synonyms: Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula: NH2CH2COOH
General description
Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.
Application
- Glycine has been used for the preparation of gelatin composites scaffold.
- It has been used in the elution buffer to extract protein using HiTrap protein G column.
- It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.
Biochem/physiol Actions
Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.
PROPERTIES
Assay
≥99%
form
powder
technique(s)
electrophoresis: suitable
western blot: suitable
impurities
≤0.01% Insoluble matter
color
white to off-white
pKa (25 °C)
(1) 2.35, (2) 9.60
2.35
mp
240 °C (dec.) (lit.)
solubility
H2O: 200 mg/mL, clear, colorless to faintly yellow
anion traces
chloride (Cl–): ≤70 ppm
cation traces
heavy metals (as Pb): ≤20 ppm
absorption
≤0.15 at 280 at 1 M
Reviews
There are no reviews yet.