Description
Application
Hektoen enteric agar is a type of primary standard media, which may be commonly used in the selective detection and isolation of Salmonella in food microbiology labs and in human stool specimens.
Preparation Note
Suspend 76.67 g in 1 litre distilled water and let it soak. Heat up by constant stirring until boiling. Cool to 55 – 60 °C and pour into sterile plates. Do not autoclave. The Medium is very thermolabile and thus overheating should be avoided.
Other Notes
Plating medium for the isolation of enteric pathogens; Isolation of Shigellae
PROPERTIES
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
acid fuchsin, 0.1 g/L
agar, 15.0 g/L
ammonium ferric citrate, 1.5 g/L
bile salts, 9 g/L
bromothymol blue, 0.065 g/L
lactose, 12 g/L
mixed peptone, 12 g/L
salicin, 2 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 5 g/L
sucrose, 12 g/L
yeast extract, 3 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
7.5±0.2 (25 °C)
application(s)
clinical testing
environmental
food and beverages
water monitoring
microbiology
suitability
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for bacteria (General Media)
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