Description
Typical Composition
Patented capture solution for sampling from Jet A1 fuel samples. Adenosine triphosphate (ATP) is detected specifically by reaction with a luciferin/luciferase reagent in buffered solution.
Features and Benefits
• Results in minutes compared to days
• De-skilled method
• Field test – no need for laboratory facilities
• Quantitative, Objective results
• Numerical read-out. Easy interpretation
• Same protocol for all jet fuel samples (with / without water)
• Same action limits for all samples (with / without water)
• Detects biological activity directly in the sample. Is not dependant on growth of microorganisms in laboratory media
• Flexible sample volume. 1 litre recommended but lower volumes can be tested. For comparison with guidelines results can be volume adjusted: RLU/litre = RLU x (1000 ml/ ml sample volume)
• Recommended by IATA guideline, 2nd Edition
Experimental Procedure
Fuel contains small amounts of water and bears risk of microbial contamination. The biomass (e.g. bacteria, fungi) may grow and cause blocking of filters or corrosion of tanks. Such damage is a very expensive issue especially in civil aviation, when leading to unplanned maintenance in airport hangars and when waiting three days for microbiology results.
Protocol: Transfer the capture solution into 1 liter fuel sample.
Close the bottle tightly, shake and let stand at least 5 minutes.
Transfer the blue capture solution back to the pen sample tube.
Dip the white stick of the pen into the liquid sample and press the stick into the Pen cuvette. Press and twist (screw) the upper part of the Pen until it contacts the lower part. Shake the Pen, then put into luminometer for measurement. Close lid and read the result on the HY-LiTE® luminometer display.
Specification
| Application | Examination of biomass contamination of jet fuel samples |
| Format | Ready prepared cuvette test format for use with HY-LiTE® 2 luminometer |
| Pipettes | Each one small and large pipette for sample transfer |
| Reagent | Contains freeze-dried and stabilized luciferin/luciferase reagent (U.S. patents 5583024, 5674713, 5700673) |
| Capture solution | Patented solution for capturing of biomass from 1 liter fuel sample |
| Detection limit | 1.4 x 10-14 mol ATP |
| Interference | Chemical additives and contaminants such as FSII and anticorrosive agents may interfere with the efficiency of the Capture Solution and the HY-LiTE® reaction and cause lower than expected readings. Biocides used for treatment of contaminated fuel may interfere with the reaction chemistry, depending on the concentration and type of biocide in the fuel. The effect of Kathon FP1.5 at 100 ppm w/ w and Biobor JF at 270 ppm w/w have been tested in fuel and cause no significant interference on the HY-LiTE® test. |
| Ambient conditions | Measurements at +5 to +35 °C |
| Storage conditions | The test pens are stable up to the date stated on the pack, when stored closed at +2 to +8°C. The shelf-life includes a period of transport or storage of up to 3 weeks at room temperature. |
| Disposal | HY-LiTE pens can be disposed off with the normal waste. |
![HY-LiTE® Hygiene Swab [Pack of 50]](https://idealmedical.co.za/wp/wp-content/uploads/2022/05/436-hylite-swab-300x300.jpg)
![HY-LiTE® Sampling Pens [Pack of 50]](https://idealmedical.co.za/wp/wp-content/uploads/2020/07/150-Hy-lite-refill-300x300.jpg)
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