Description
Synonym: LB Agar
General description
LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7.
Application
LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.
Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.
Preparation Note
Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.
PROPERTIES
sterility
non-sterile
Pack Size
500g
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15 g/L
casein enzymic hydrolysate, 10 g/L
sodium chloride, 10 g/L
yeast extract, 5 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
7.5±0.2 (25 °C)
application(s)
clinical testing
food and beverages
life science and biopharma
microbiology
suitability
nonselective for Escherichia coli
nonselective for bacteria (General Media)
nonselective for coliforms
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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